Field of view advantage of conjugate adaptive optics in microscopy applications

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Field of view advantage of conjugate adaptive optics in microscopy applications.

The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases the DM is placed conjugate to the microscope pupil, called pupil adaptive optics (AO). When the aberrations are spatially variant an alternative configuration invol...

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We describe an adaptive optics technique for two-photon microscopy in which the deformable mirror used for aberration compensation is positioned in a plane conjugate to the plane of the aberration. We demonstrate in a proof-of-principle experiment that this technique yields a large field of view advantage in comparison to standard pupil-conjugate adaptive optics. Further, we show that the exten...

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ژورنال

عنوان ژورنال: Applied Optics

سال: 2015

ISSN: 0003-6935,1539-4522

DOI: 10.1364/ao.54.003498